E coli strains
Laboratory bacteria are divided into two main strains: expression strains and cloning strains. Expression strains are optimised for protein yield, while cloning strains are optimised for maintaining a high copy of plasmids.
Cloning strains
Cloning strains
XL1 blue (Tetr)
XL10 gold (Chlorr)
DH5alpha
Expression strains
Expression strains
BL21
C41
Rosetta (for proteins with rare codon usage)
Rosetta-gami (enhanced expression for eukaryotic proteins with rare codon usage)