Coomassie stain
Stain
Stain
45.4% ethanol
9.2% acetic acid
45.2% water
1g in 1l of Coomassie blue (Brilliant Blue R) - a tiny dash
Stir well (at least 1hr) and filter before use
Destain
Destain
5% ethanol
7.5% acetic acid
87.5% water
45.4% ethanol
9.2% acetic acid
45.2% water
1g in 1l of Coomassie blue (Brilliant Blue R) - a tiny dash
Stir well (at least 1hr) and filter before use
5% ethanol
7.5% acetic acid
87.5% water