Gel sample buffer

Agarose sample buffer

50% glycerol

50% water

0.04% bromophenol blue (or until looks OK)

Use 2ul buffer with 10ul sample

2x SDS PAGE sample buffer

100mM tris pH6.8

200mM DTT (or 10% BME)

4% SDS (electrophoresis grade)

0.2% bromophenol blue

20% glycerol

If storing at room temperature, omit reducing agent and add just prior to use.