Biochemistry
Bioinformatics Tools
Multiple Sequence Alignments from EBI
Reverse and complement a DNA sequence
NEB Restriction Digests Calculator
Plasmapper for drawing maps of plasmids
Finnzymes Tm calculator for primers
Clontech In-fusion primer calculator
Quikchange primer design site
ProtParam protein parameter calculator
RONN protein disorder predictor
SWISS-MODEL protein structure homology-modeling
Pubmed literature database
Useful ordering websites
Place a sequencing order with Source BioScience.
Source BioScience requires at least 5ul DNA (100ng/ul for plasmids, 1ng/ul per 100bp for PCR products). If you are sending your own primers, they require 10ul of 3.2pmol/ul. Source recommends that primers that are continuing from a previous sequencing run should allow 100bp overlap to be safe and complete the coverage and definitely no less than a 50bp overlap.
The customer service phone number for the Oxford lab is 01865 275 236. They can be e-mailed for technical support on sequencing.oxford@sourcebioscience.com.
Order oligonucleotides from MWG (Eurofins)